MiR156 regulates anthocyanin biosynthesis through SPL targets and other microRNAs in poplar.
Identifieur interne : 000272 ( Main/Exploration ); précédent : 000271; suivant : 000273MiR156 regulates anthocyanin biosynthesis through SPL targets and other microRNAs in poplar.
Auteurs : Yamei Wang [République populaire de Chine] ; Wenwen Liu [République populaire de Chine] ; Xinwei Wang [République populaire de Chine] ; Ruijuan Yang [République populaire de Chine] ; Zhenying Wu [République populaire de Chine] ; Han Wang [République populaire de Chine] ; Lei Wang [République populaire de Chine] ; Zhubing Hu [République populaire de Chine] ; Siyi Guo [République populaire de Chine] ; Hailing Zhang ; Jinxing Lin [République populaire de Chine] ; Chunxiang Fu [République populaire de Chine]Source :
- Horticulture research [ 2052-7276 ] ; 2020.
Abstract
Anthocyanins biosynthesized from the flavonoid pathway are types of pigments that are involved in the protection of poplar from biotic and abiotic stresses. Previous researchers studying anthocyanin-related transcription factors and structural genes in poplar have made significant discoveries. However, little is known about the regulatory role of microRNAs in anthocyanin biosynthesis in poplar. Here, we overexpressed miR156 in poplar to study the comprehensive effects of the miR156-SPL module on the biosynthesis of anthocyanins. Small RNA sequencing analysis revealed 228 microRNAs differentially expressed in transgenic poplar plants with dramatically increased miR156 levels. Furthermore, integrated microRNAomic and transcriptomic analysis suggested that two microRNAs, miR160h, and miR858, have the potential to affect anthocyanin accumulation in poplar by regulating auxin response factors and MYB transcription factors, respectively. Additionally, the accumulation of miR160h and miR858 displayed a positive correlation with miR156 levels, suggesting a possible interaction between the miR156-SPL module and these microRNAs in poplar. Last, metabolomics analysis revealed that the levels of anthocyanins, flavones, and flavonols were substantially elevated in transgenic poplar plants overexpressing miR156 compared with the wild type, whereas the total lignin content was reduced in the transgenic plants. Taken together, our results indicate that miR156 can fine tune the anthocyanin biosynthetic pathway via multiple factors, including microRNAs, transcription factors, and the levels of structural genes, in poplar. This provides additional clues for understanding the complex regulatory network of anthocyanin biosynthesis in woody plants.
DOI: 10.1038/s41438-020-00341-w
PubMed: 32821401
PubMed Central: PMC7395715
Affiliations:
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targets and other microRNAs in poplar.</title><author><name sortKey="Wang, Yamei" sort="Wang, Yamei" uniqKey="Wang Y" first="Yamei" last="Wang">Yamei Wang</name><affiliation wicri:level="1"><nlm:affiliation>Shandong Provincial Key Laboratory of Energy Genetics, Key Laboratory of Biofuels, Chinese Academy of Sciences, Qingdao Institute of BioEnergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, 266101 China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Shandong Provincial Key Laboratory of Energy Genetics, Key Laboratory of Biofuels, Chinese Academy of Sciences, Qingdao Institute of BioEnergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao</wicri:regionArea><wicri:noRegion>Qingdao</wicri:noRegion></affiliation><affiliation wicri:level="3"><nlm:affiliation>University of Chinese Academy of Sciences, Beijing, 100049 China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>University of Chinese Academy of Sciences, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Liu, Wenwen" sort="Liu, Wenwen" uniqKey="Liu W" first="Wenwen" last="Liu">Wenwen Liu</name><affiliation wicri:level="1"><nlm:affiliation>Shandong Provincial Key Laboratory of Energy Genetics, Key Laboratory of Biofuels, Chinese Academy of Sciences, Qingdao Institute of BioEnergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, 266101 China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Shandong Provincial Key Laboratory of Energy Genetics, Key Laboratory of Biofuels, Chinese Academy of Sciences, Qingdao Institute of BioEnergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao</wicri:regionArea><wicri:noRegion>Qingdao</wicri:noRegion></affiliation></author><author><name sortKey="Wang, Xinwei" sort="Wang, Xinwei" uniqKey="Wang X" first="Xinwei" last="Wang">Xinwei Wang</name><affiliation wicri:level="3"><nlm:affiliation>College of Biological Sciences & Biotechnology, Beijing Forestry University, Beijing, 10083 China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Biological Sciences & Biotechnology, Beijing Forestry University, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Yang, Ruijuan" sort="Yang, Ruijuan" uniqKey="Yang R" first="Ruijuan" last="Yang">Ruijuan Yang</name><affiliation wicri:level="1"><nlm:affiliation>Shandong Provincial Key Laboratory of Energy Genetics, Key Laboratory of Biofuels, Chinese Academy of Sciences, Qingdao Institute of BioEnergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, 266101 China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Shandong Provincial Key Laboratory of Energy Genetics, Key Laboratory of Biofuels, Chinese Academy of Sciences, Qingdao Institute of BioEnergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao</wicri:regionArea><wicri:noRegion>Qingdao</wicri:noRegion></affiliation><affiliation wicri:level="3"><nlm:affiliation>University of Chinese Academy of Sciences, Beijing, 100049 China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>University of Chinese Academy of Sciences, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Wu, Zhenying" sort="Wu, Zhenying" uniqKey="Wu Z" first="Zhenying" last="Wu">Zhenying Wu</name><affiliation wicri:level="1"><nlm:affiliation>Shandong Provincial Key Laboratory of Energy Genetics, Key Laboratory of Biofuels, Chinese Academy of Sciences, Qingdao Institute of BioEnergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, 266101 China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Shandong Provincial Key Laboratory of Energy Genetics, Key Laboratory of Biofuels, Chinese Academy of Sciences, Qingdao Institute of BioEnergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao</wicri:regionArea><wicri:noRegion>Qingdao</wicri:noRegion></affiliation></author><author><name sortKey="Wang, Han" sort="Wang, Han" uniqKey="Wang H" first="Han" last="Wang">Han Wang</name><affiliation wicri:level="1"><nlm:affiliation>Shandong Provincial Key Laboratory of Energy Genetics, Key Laboratory of Biofuels, Chinese Academy of Sciences, Qingdao Institute of BioEnergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, 266101 China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Shandong Provincial Key Laboratory of Energy Genetics, Key Laboratory of Biofuels, Chinese Academy of Sciences, Qingdao Institute of BioEnergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao</wicri:regionArea><wicri:noRegion>Qingdao</wicri:noRegion></affiliation><affiliation wicri:level="3"><nlm:affiliation>University of Chinese Academy of Sciences, Beijing, 100049 China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>University of Chinese Academy of Sciences, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Wang, Lei" sort="Wang, Lei" uniqKey="Wang L" first="Lei" last="Wang">Lei Wang</name><affiliation wicri:level="1"><nlm:affiliation>Collaborative Innovation Center of Crop Stress Biology, Henan Province and Institute of Plant Stress Biology, Henan University, Kaifeng, 475001 China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Collaborative Innovation Center of Crop Stress Biology, Henan Province and Institute of Plant Stress Biology, Henan University, Kaifeng</wicri:regionArea><wicri:noRegion>Kaifeng</wicri:noRegion></affiliation></author><author><name sortKey="Hu, Zhubing" sort="Hu, Zhubing" uniqKey="Hu Z" first="Zhubing" last="Hu">Zhubing Hu</name><affiliation wicri:level="1"><nlm:affiliation>Collaborative Innovation Center of Crop Stress Biology, Henan Province and Institute of Plant Stress Biology, Henan University, Kaifeng, 475001 China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Collaborative Innovation Center of Crop Stress Biology, Henan Province and Institute of Plant Stress Biology, Henan University, Kaifeng</wicri:regionArea><wicri:noRegion>Kaifeng</wicri:noRegion></affiliation></author><author><name sortKey="Guo, Siyi" sort="Guo, Siyi" uniqKey="Guo S" first="Siyi" last="Guo">Siyi Guo</name><affiliation wicri:level="1"><nlm:affiliation>Collaborative Innovation Center of Crop Stress Biology, Henan Province and Institute of Plant Stress Biology, Henan University, Kaifeng, 475001 China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Collaborative Innovation Center of Crop Stress Biology, Henan Province and Institute of Plant Stress Biology, Henan University, Kaifeng</wicri:regionArea><wicri:noRegion>Kaifeng</wicri:noRegion></affiliation></author><author><name sortKey="Zhang, Hailing" sort="Zhang, Hailing" uniqKey="Zhang H" first="Hailing" last="Zhang">Hailing Zhang</name><affiliation><nlm:affiliation>Grass and Science Institute of Heilongjiang Academy of Agricultural Sciences, Harbin, Heilongjiang China.</nlm:affiliation><wicri:noCountry code="subField">Heilongjiang China</wicri:noCountry></affiliation></author><author><name sortKey="Lin, Jinxing" sort="Lin, Jinxing" uniqKey="Lin J" first="Jinxing" last="Lin">Jinxing Lin</name><affiliation wicri:level="3"><nlm:affiliation>College of Biological Sciences & Biotechnology, Beijing Forestry University, Beijing, 10083 China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Biological Sciences & Biotechnology, Beijing Forestry University, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Fu, Chunxiang" sort="Fu, Chunxiang" uniqKey="Fu C" first="Chunxiang" last="Fu">Chunxiang Fu</name><affiliation wicri:level="1"><nlm:affiliation>Shandong Provincial Key Laboratory of Energy Genetics, Key Laboratory of Biofuels, Chinese Academy of Sciences, Qingdao Institute of BioEnergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, 266101 China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Shandong Provincial Key Laboratory of Energy Genetics, Key Laboratory of Biofuels, Chinese Academy of Sciences, Qingdao Institute of BioEnergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao</wicri:regionArea><wicri:noRegion>Qingdao</wicri:noRegion></affiliation></author></analytic><series><title level="j">Horticulture research</title><idno type="ISSN">2052-7276</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Anthocyanins biosynthesized from the flavonoid pathway are types of pigments that are involved in the protection of poplar from biotic and abiotic stresses. Previous researchers studying anthocyanin-related transcription factors and structural genes in poplar have made significant discoveries. However, little is known about the regulatory role of microRNAs in anthocyanin biosynthesis in poplar. Here, we overexpressed miR156 in poplar to study the comprehensive effects of the miR156-<i>SPL</i> module on the biosynthesis of anthocyanins. Small RNA sequencing analysis revealed 228 microRNAs differentially expressed in transgenic poplar plants with dramatically increased miR156 levels. Furthermore, integrated microRNAomic and transcriptomic analysis suggested that two microRNAs, miR160h, and miR858, have the potential to affect anthocyanin accumulation in poplar by regulating auxin response factors and MYB transcription factors, respectively. Additionally, the accumulation of miR160h and miR858 displayed a positive correlation with miR156 levels, suggesting a possible interaction between the miR156-<i>SPL</i> module and these microRNAs in poplar. Last, metabolomics analysis revealed that the levels of anthocyanins, flavones, and flavonols were substantially elevated in transgenic poplar plants overexpressing miR156 compared with the wild type, whereas the total lignin content was reduced in the transgenic plants. Taken together, our results indicate that miR156 can fine tune the anthocyanin biosynthetic pathway via multiple factors, including microRNAs, transcription factors, and the levels of structural genes, in poplar. This provides additional clues for understanding the complex regulatory network of anthocyanin biosynthesis in woody plants.</div></front></TEI><pubmed><MedlineCitation Status="PubMed-not-MEDLINE" Owner="NLM"><PMID Version="1">32821401</PMID><DateRevised><Year>2020</Year><Month>09</Month><Day>28</Day></DateRevised><Article PubModel="Electronic-eCollection"><Journal><ISSN IssnType="Print">2052-7276</ISSN><JournalIssue CitedMedium="Print"><Volume>7</Volume><PubDate><Year>2020</Year></PubDate></JournalIssue><Title>Horticulture research</Title><ISOAbbreviation>Hortic Res</ISOAbbreviation></Journal><ArticleTitle>MiR156 regulates anthocyanin biosynthesis through <i>SPL</i> targets and other microRNAs in poplar.</ArticleTitle><Pagination><MedlinePgn>118</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1038/s41438-020-00341-w</ELocationID><Abstract><AbstractText>Anthocyanins biosynthesized from the flavonoid pathway are types of pigments that are involved in the protection of poplar from biotic and abiotic stresses. Previous researchers studying anthocyanin-related transcription factors and structural genes in poplar have made significant discoveries. However, little is known about the regulatory role of microRNAs in anthocyanin biosynthesis in poplar. Here, we overexpressed miR156 in poplar to study the comprehensive effects of the miR156-<i>SPL</i> module on the biosynthesis of anthocyanins. Small RNA sequencing analysis revealed 228 microRNAs differentially expressed in transgenic poplar plants with dramatically increased miR156 levels. Furthermore, integrated microRNAomic and transcriptomic analysis suggested that two microRNAs, miR160h, and miR858, have the potential to affect anthocyanin accumulation in poplar by regulating auxin response factors and MYB transcription factors, respectively. Additionally, the accumulation of miR160h and miR858 displayed a positive correlation with miR156 levels, suggesting a possible interaction between the miR156-<i>SPL</i> module and these microRNAs in poplar. Last, metabolomics analysis revealed that the levels of anthocyanins, flavones, and flavonols were substantially elevated in transgenic poplar plants overexpressing miR156 compared with the wild type, whereas the total lignin content was reduced in the transgenic plants. Taken together, our results indicate that miR156 can fine tune the anthocyanin biosynthetic pathway via multiple factors, including microRNAs, transcription factors, and the levels of structural genes, in poplar. This provides additional clues for understanding the complex regulatory network of anthocyanin biosynthesis in woody plants.</AbstractText><CopyrightInformation>© The Author(s) 2020.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y" EqualContrib="Y"><LastName>Wang</LastName><ForeName>Yamei</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>Shandong Provincial Key Laboratory of Energy Genetics, Key Laboratory of Biofuels, Chinese Academy of Sciences, Qingdao Institute of BioEnergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, 266101 China.</Affiliation><Identifier Source="GRID">grid.458500.c</Identifier><Identifier Source="ISNI">0000 0004 1806 7609</Identifier></AffiliationInfo><AffiliationInfo><Affiliation>University of Chinese Academy of Sciences, Beijing, 100049 China.</Affiliation><Identifier Source="GRID">grid.410726.6</Identifier><Identifier Source="ISNI">0000 0004 1797 8419</Identifier></AffiliationInfo></Author><Author ValidYN="Y" EqualContrib="Y"><LastName>Liu</LastName><ForeName>Wenwen</ForeName><Initials>W</Initials><AffiliationInfo><Affiliation>Shandong Provincial Key Laboratory of Energy Genetics, Key Laboratory of Biofuels, Chinese Academy of Sciences, Qingdao Institute of BioEnergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, 266101 China.</Affiliation><Identifier Source="GRID">grid.458500.c</Identifier><Identifier Source="ISNI">0000 0004 1806 7609</Identifier></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Xinwei</ForeName><Initials>X</Initials><AffiliationInfo><Affiliation>College of Biological Sciences & Biotechnology, Beijing Forestry University, Beijing, 10083 China.</Affiliation><Identifier Source="GRID">grid.66741.32</Identifier><Identifier Source="ISNI">0000 0001 1456 856X</Identifier></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Yang</LastName><ForeName>Ruijuan</ForeName><Initials>R</Initials><AffiliationInfo><Affiliation>Shandong Provincial Key Laboratory of Energy Genetics, Key Laboratory of Biofuels, Chinese Academy of Sciences, Qingdao Institute of BioEnergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, 266101 China.</Affiliation><Identifier Source="GRID">grid.458500.c</Identifier><Identifier Source="ISNI">0000 0004 1806 7609</Identifier></AffiliationInfo><AffiliationInfo><Affiliation>University of Chinese Academy of Sciences, Beijing, 100049 China.</Affiliation><Identifier Source="GRID">grid.410726.6</Identifier><Identifier Source="ISNI">0000 0004 1797 8419</Identifier></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wu</LastName><ForeName>Zhenying</ForeName><Initials>Z</Initials><AffiliationInfo><Affiliation>Shandong Provincial Key Laboratory of Energy Genetics, Key Laboratory of Biofuels, Chinese Academy of Sciences, Qingdao Institute of BioEnergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, 266101 China.</Affiliation><Identifier Source="GRID">grid.458500.c</Identifier><Identifier Source="ISNI">0000 0004 1806 7609</Identifier></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Han</ForeName><Initials>H</Initials><AffiliationInfo><Affiliation>Shandong Provincial Key Laboratory of Energy Genetics, Key Laboratory of Biofuels, Chinese Academy of Sciences, Qingdao Institute of BioEnergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, 266101 China.</Affiliation><Identifier Source="GRID">grid.458500.c</Identifier><Identifier Source="ISNI">0000 0004 1806 7609</Identifier></AffiliationInfo><AffiliationInfo><Affiliation>University of Chinese Academy of Sciences, Beijing, 100049 China.</Affiliation><Identifier Source="GRID">grid.410726.6</Identifier><Identifier Source="ISNI">0000 0004 1797 8419</Identifier></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Lei</ForeName><Initials>L</Initials><AffiliationInfo><Affiliation>Collaborative Innovation Center of Crop Stress Biology, Henan Province and Institute of Plant Stress Biology, Henan University, Kaifeng, 475001 China.</Affiliation><Identifier Source="GRID">grid.256922.8</Identifier><Identifier Source="ISNI">0000 0000 9139 560X</Identifier></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Hu</LastName><ForeName>Zhubing</ForeName><Initials>Z</Initials><AffiliationInfo><Affiliation>Collaborative Innovation Center of Crop Stress Biology, Henan Province and Institute of Plant Stress Biology, Henan University, Kaifeng, 475001 China.</Affiliation><Identifier Source="GRID">grid.256922.8</Identifier><Identifier Source="ISNI">0000 0000 9139 560X</Identifier></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Guo</LastName><ForeName>Siyi</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Collaborative Innovation Center of Crop Stress Biology, Henan Province and Institute of Plant Stress Biology, Henan University, Kaifeng, 475001 China.</Affiliation><Identifier Source="GRID">grid.256922.8</Identifier><Identifier Source="ISNI">0000 0000 9139 560X</Identifier></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zhang</LastName><ForeName>Hailing</ForeName><Initials>H</Initials><AffiliationInfo><Affiliation>Grass and Science Institute of Heilongjiang Academy of Agricultural Sciences, Harbin, Heilongjiang China.</Affiliation><Identifier Source="GRID">grid.452609.c</Identifier></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Lin</LastName><ForeName>Jinxing</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>College of Biological Sciences & Biotechnology, Beijing Forestry University, Beijing, 10083 China.</Affiliation><Identifier Source="GRID">grid.66741.32</Identifier><Identifier Source="ISNI">0000 0001 1456 856X</Identifier></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Fu</LastName><ForeName>Chunxiang</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>Shandong Provincial Key Laboratory of Energy Genetics, Key Laboratory of Biofuels, Chinese Academy of Sciences, Qingdao Institute of BioEnergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, 266101 China.</Affiliation><Identifier Source="GRID">grid.458500.c</Identifier><Identifier Source="ISNI">0000 0004 1806 7609</Identifier></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2020</Year><Month>08</Month><Day>01</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Hortic Res</MedlineTA><NlmUniqueID>101655540</NlmUniqueID><ISSNLinking>2052-7276</ISSNLinking></MedlineJournalInfo><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Molecular engineering in plants</Keyword><Keyword MajorTopicYN="N">Secondary metabolism</Keyword></KeywordList><CoiStatement>Conflict of interestThe authors declare that they have no conflict of interest.</CoiStatement></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2020</Year><Month>02</Month><Day>28</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2020</Year><Month>04</Month><Day>23</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2020</Year><Month>05</Month><Day>12</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2020</Year><Month>8</Month><Day>22</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2020</Year><Month>8</Month><Day>22</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2020</Year><Month>8</Month><Day>22</Day><Hour>6</Hour><Minute>1</Minute></PubMedPubDate></History><PublicationStatus>epublish</PublicationStatus><ArticleIdList><ArticleId 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Chine</li></country><settlement><li>Pékin</li></settlement></list><tree><noCountry><name sortKey="Zhang, Hailing" sort="Zhang, Hailing" uniqKey="Zhang H" first="Hailing" last="Zhang">Hailing Zhang</name></noCountry><country name="République populaire de Chine"><noRegion><name sortKey="Wang, Yamei" sort="Wang, Yamei" uniqKey="Wang Y" first="Yamei" last="Wang">Yamei Wang</name></noRegion><name sortKey="Fu, Chunxiang" sort="Fu, Chunxiang" uniqKey="Fu C" first="Chunxiang" last="Fu">Chunxiang Fu</name><name sortKey="Guo, Siyi" sort="Guo, Siyi" uniqKey="Guo S" first="Siyi" last="Guo">Siyi Guo</name><name sortKey="Hu, Zhubing" sort="Hu, Zhubing" uniqKey="Hu Z" first="Zhubing" last="Hu">Zhubing Hu</name><name sortKey="Lin, Jinxing" sort="Lin, Jinxing" uniqKey="Lin J" first="Jinxing" last="Lin">Jinxing Lin</name><name sortKey="Liu, Wenwen" sort="Liu, Wenwen" uniqKey="Liu W" first="Wenwen" last="Liu">Wenwen Liu</name><name sortKey="Wang, Han" sort="Wang, Han" uniqKey="Wang H" first="Han" last="Wang">Han Wang</name><name sortKey="Wang, Han" sort="Wang, Han" uniqKey="Wang H" first="Han" last="Wang">Han Wang</name><name sortKey="Wang, Lei" sort="Wang, Lei" uniqKey="Wang L" first="Lei" last="Wang">Lei Wang</name><name sortKey="Wang, Xinwei" sort="Wang, Xinwei" uniqKey="Wang X" first="Xinwei" last="Wang">Xinwei Wang</name><name sortKey="Wang, Yamei" sort="Wang, Yamei" uniqKey="Wang Y" first="Yamei" last="Wang">Yamei Wang</name><name sortKey="Wu, Zhenying" sort="Wu, Zhenying" uniqKey="Wu Z" first="Zhenying" last="Wu">Zhenying Wu</name><name sortKey="Yang, Ruijuan" sort="Yang, Ruijuan" uniqKey="Yang R" first="Ruijuan" last="Yang">Ruijuan Yang</name><name sortKey="Yang, Ruijuan" sort="Yang, Ruijuan" uniqKey="Yang R" first="Ruijuan" last="Yang">Ruijuan Yang</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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